What key observational difference, related to chemical signatures, can help distinguish late $ ext{M}$-types from the hottest brown dwarfs?

Answer

The presence or absence of lithium

Since stars rapidly deplete lithium during hydrogen fusion, a positive lithium signature often indicates that an object is a brown dwarf that never achieved full stellar status.

What key observational difference, related to chemical signatures, can help distinguish late $	ext{M}$-types from the hottest brown dwarfs?
astronomytemperaturestarmain-sequence